抗（1R，2R）-环己二胺铂（II）-DNA加合物单克隆抗体的鉴定

Characterization of monoclonal antibodies against (1R,2R)-cyclohexanediamine platinum(II)-DNA adduct.

作者信息

Hirose J, Inoue K, Morimoto E, Iwamoto H, Yamaguti Y, Kitase M, Inagaki K, Hiromi K

机构信息

Department of Food Science and Technology, Faculty of Engineering, Fukuyama University, Japan.

出版信息

Biol Pharm Bull. 1996 Sep;19(9):1220-2. doi: 10.1248/bpb.19.1220.

DOI:10.1248/bpb.19.1220

PMID:8889045

Abstract

Two monoclonal antibodies raised against Pt(II)(1R,2R-cyclohexanediamine)-DNA were prepared, and the specificity of the monoclonal antibodies against Pt(II)(cyclohexanediamine)-DNA derivatives was determined by competitive enzyme-linked immunosorbent assay. The binding affinity of the monoclonal antibodies is apparently influenced by the cyclohexanediamine moiety of Pt(II)(cyclohexanediamine)-DNA adducts, but the monoclonal antibodies can not bind to the low molecular analogue, Pt(II)(1R,2R-cyclohexanediamine)-d(GpG), which is the intrastrand binding model compound of Pt(II)(1R,2R-cyclohexanediamine)-DNA. Therefore, the monoclonal antibodies recognize the macromolecular parts, including DNA duplex, in addition to the cyclohexane moieties of the platinum complexes on Pt(II)(cyclohexanediamine)-DNA adducts.

摘要

制备了两种针对Pt(II)(1R,2R - 环己二胺)-DNA的单克隆抗体，并通过竞争性酶联免疫吸附测定法确定了这些单克隆抗体对Pt(II)(环己二胺)-DNA衍生物的特异性。单克隆抗体的结合亲和力显然受到Pt(II)(环己二胺)-DNA加合物中环己二胺部分的影响，但这些单克隆抗体不能与低分子类似物Pt(II)(1R,2R - 环己二胺)-d(GpG)结合，后者是Pt(II)(1R,2R - 环己二胺)-DNA的链内结合模型化合物。因此，这些单克隆抗体除了识别Pt(II)(环己二胺)-DNA加合物上铂配合物的环己烷部分外，还能识别包括DNA双链体在内的大分子部分。