Furukawa M, Fukuda Y, Tashiro H, Ohdan H, Hoshino S, Shintaku S, Itou H, Kiyohiko D
Second Department of Surgery, Hiroshima University School of Medicine, Japan.
Cell Transplant. 1996 Sep-Oct;5(5 Suppl 1):S75-7. doi: 10.1016/0963-6897(96)00045-0.
Intrathymic injection of donor alloantigens (splenocytes) was performed in rat heart transplantation to induced tolerance. In our experimental protocol, male (BN rats, RT1n) heart allografts were transplanted to female (LEW rats, RT1(1)) recipients in order to detect the existence of Y-chromosomes in recipients' tissues using the polymerase chain reaction (PCR) after the establishment of microchimerism. The mean survival time (MST) of male heart allografts was prolonged by the thymic injection protocol. PCR analysis of the Y-chromosomes of these recipients showed that splenocytes inoculated into the thymus still remained after as long as 30 days, while none of the cells that originated from the donor were never detected in the peripheral blood. On the other hand, our previous study demonstrated that the donor-specific Y-chromosomes could be detected in all the tissues of the females (peripheral blood, lymph nodes, spleen, and liver) except for thymus, of the sex-mismatched rat liver transplantation without thymic injection. Completely inconsistent results were deduced from two of our experiments, but the present study demonstrated that thymic clonal deletion played a major role in the prolongation of allograft survival after intrathymic injection of donor alloantigens.
在大鼠心脏移植中进行胸腺内注射供体同种异体抗原（脾细胞）以诱导耐受。在我们的实验方案中，将雄性（BN大鼠，RT1n）心脏异体移植物移植到雌性（LEW大鼠，RT1(1)）受体中，以便在微嵌合体形成后使用聚合酶链反应（PCR）检测受体组织中Y染色体的存在。胸腺注射方案延长了雄性心脏异体移植物的平均存活时间（MST）。对这些受体的Y染色体进行PCR分析表明，注入胸腺的脾细胞在长达30天后仍有残留，而在外周血中从未检测到来自供体的细胞。另一方面，我们之前的研究表明，在未进行胸腺注射的性别不匹配大鼠肝移植中，除胸腺外，在雌性（外周血、淋巴结、脾脏和肝脏）的所有组织中都能检测到供体特异性Y染色体。我们的两个实验得出了完全不一致的结果，但本研究表明，胸腺克隆清除在胸腺内注射供体同种异体抗原后延长移植物存活中起主要作用。
