在编码一种GTP结合蛋白的cDNA表达后脂多糖介导的B细胞反应的恢复
Restoration of lipopolysaccharide-mediated B-cell response after expression of a cDNA encoding a GTP-binding protein.
作者信息
Kang A D, Wong P M, Chen H, Castagna R, Chung S W, Sultzer B M
机构信息
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA.
出版信息
Infect Immun. 1996 Nov;64(11):4612-7. doi: 10.1128/iai.64.11.4612-4617.1996.
Abstract
Previous analysis of hybrid progeny derived from lipopolysaccharide (LPS) responder and nonresponder inbred mouse strains demonstrated that a single genetic locus controlled responsiveness to LPS. Using a differential functional screening approach, we report the isolation of a cDNA that has sequence homology to a GTP-binding protein. Expression of the cDNA in splenic B cells of C3H/HeJ nonresponder, endotoxin-resistant mice resulted in polyclonal B-cell activation in response to LPS stimulation. Thus a GTP-binding protein may be involved in LPS stimulation in B cells and perhaps other cell types.
摘要
先前对来自脂多糖（LPS）应答和不应答近交小鼠品系的杂交后代进行的分析表明，单个基因座控制对LPS的反应性。使用差异功能筛选方法，我们报告分离出一种与GTP结合蛋白具有序列同源性的cDNA。该cDNA在C3H/HeJ不应答、内毒素抗性小鼠的脾B细胞中表达，导致对LPS刺激产生多克隆B细胞活化。因此，一种GTP结合蛋白可能参与B细胞以及也许其他细胞类型中的LPS刺激。
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