细胞培养中海兔感觉运动突触的长期抑制：突触后钙升高的诱导作用

Long-term depression of Aplysia sensorimotor synapses in cell culture: inductive role of a rise in postsynaptic calcium.

Lin X Y, Glanzman D L

Department of Physiological Science, University of California, Los Angeles 90095, USA.

J Neurophysiol. 1996 Sep;76(3):2111-4. doi: 10.1152/jn.1996.76.3.2111.

Abstract

Activation of sensory neurons at 2 Hz for 15 min induces long-term depression (LTD) of isolated Aplysia sensorimotor synapses in cell culture. 2. Prior infusion of the Ca2+ chelator 1,2-bis-(2-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA) into the postsynaptic motor neuron blocks the induction of LTD, but not short-term synaptic depression. 3. Invertebrate central synapses possess the capacity for LTD. This form of long-term synaptic plasticity may play an important role in learning in Aplysia.

摘要

以2赫兹的频率激活感觉神经元15分钟，可诱导细胞培养中分离的海兔感觉运动突触产生长时程抑制（LTD）。2. 事先将Ca2+螯合剂1,2-双（2-氨基苯氧基）乙烷-N,N,N',N'-四乙酸（BAPTA）注入突触后运动神经元，可阻断LTD的诱导，但不影响短时程突触抑制。3. 无脊椎动物的中枢突触具有产生LTD的能力。这种形式的长期突触可塑性可能在海兔的学习中发挥重要作用。