Nikolaidis N, Scouras Z G
Department of Genetics, Development and Molecular Biology, School of Biology, Faculty of Science, Aristotle University of Thessaloniki, Greece.
Genome. 1996 Oct;39(5):874-83. doi: 10.1139/g96-110.
Mitochondrial DNA (mtDNA) restriction site maps for three Drosophila montium subgroup species of the melanogaster species group, inhabiting Indian and Afrotropical montium subgroup territories, were established. Taking into account previous mtDNA data concerning six oriental montium species, a phylogeny was established using distance-matrix and parsimony methods. Both genetic diversity and mtDNA size variations were found to be very narrow, suggesting close phylogenetic relationships among all montium species studied. The phylogenetic trees that were constructed revealed three main lineages for the montium subgroup species studied: one consisting of the Afrotropical species Drosophila seguyi, which is placed distantly from the other species, one comprising the north-oriental (Palearctic) species, and one comprising the southwestern (south-oriental, Australasian, Indian, and Afrotropical) species. The combination of the mtDNA data presented here with data from other species belonging to the melanogaster and obscura subgroups revealed two major clusters: melanogaster and obscura. The melanogaster cluster is further divided into two compact lineages, comprising the montium subgroup species and the melanogaster complex species; the species of the other complex of the melanogaster subgroup, yakuba, disperse among the obscura species. The above grouping is in agreement with the mtDNA size variations of the species. Overall, among all subgroups studied, the species of the montium subgroup seem to be the most closely related.
构建了栖息于印度和热带非洲山地亚组区域的黑腹果蝇种组中三种山地果蝇亚组物种的线粒体DNA（mtDNA）限制性酶切图谱。结合先前关于六种东方山地果蝇物种的mtDNA数据，使用距离矩阵法和简约法构建了系统发育树。研究发现，遗传多样性和mtDNA大小变异都非常小，这表明所有研究的山地果蝇物种之间存在密切的系统发育关系。构建的系统发育树显示，所研究的山地果蝇亚组物种有三个主要谱系：一个由热带非洲物种塞氏果蝇组成，它与其他物种的亲缘关系较远；一个由北方（古北区）物种组成；一个由西南（南方、澳大利亚、印度和热带非洲）物种组成。本文呈现的mtDNA数据与黑腹果蝇亚组和暗果蝇亚组其他物种的数据相结合，揭示了两个主要类群：黑腹果蝇类群和暗果蝇类群。黑腹果蝇类群进一步分为两个紧密的谱系，包括山地果蝇亚组物种和黑腹果蝇复合种；黑腹果蝇亚组的另一个复合种雅库布果蝇则分散在暗果蝇物种之中。上述分组与物种的mtDNA大小变异一致。总体而言,在所研究的所有亚组中，山地果蝇亚组的物种似乎亲缘关系最为密切。
