草生欧文氏菌生物操纵子的分离与特性分析以及bioA和bioB基因的序列

Isolation and characterization of the Erwinia herbicola bio operon and the sequences of the bioA and bioB genes.

作者信息

Wu C H, Chen H Y, Shiuan D

机构信息

Department of Biology, National Sun Yat-Sen University, Kaohsiung, Taiwan, ROC.

出版信息

Gene. 1996 Oct 3;174(2):251-8. doi: 10.1016/0378-1119(96)00090-x.

DOI:10.1016/0378-1119(96)00090-x

PMID:8890743

Abstract

The biotin operon of Erwinia herbicola was cloned and characterized. The operon consists of five genes arranged in the order, bioABFCD. The operon is negatively regulated via the interaction of a proposed biotin repressor with an operator sequence that lies between the bioA and bioB genes. The nucleotide sequences of bioA (7,8-diaminopelargonic acid transferase), bioB (biotin synthetase) and the regulatory region were determined and analyzed. The deduced amino acid sequences of bioA and bioB are also aligned with currently available homologs to obtain the UPGMA (unweighted pair group method with arithmetic mean) evolutionary tree.

摘要

克隆并鉴定了草生欧文氏菌的生物素操纵子。该操纵子由五个基因组成，排列顺序为bioABFCD。该操纵子通过一种假定的生物素阻遏物与位于bioA和bioB基因之间的操纵序列相互作用而受到负调控。测定并分析了bioA（7,8-二氨基壬酸转移酶）、bioB（生物素合成酶）的核苷酸序列以及调控区域。还将bioA和bioB推导的氨基酸序列与目前可得的同源物进行比对，以获得UPGMA（算术平均非加权配对组方法）进化树。