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用于粟酒裂殖酵母his3缺陷菌株的载体构建及基因组文库构建。

Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.

作者信息

Ohi R, Feoktistova A, Gould K L

机构信息

Howard Hughes Medical Institute, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA.

出版信息

Gene. 1996 Oct 3;174(2):315-8. doi: 10.1016/0378-1119(96)00085-6.

DOI:10.1016/0378-1119(96)00085-6
PMID:8890754
Abstract

The construction of vectors for use in Schizosaccharomyces pombe using the his3+ gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3(+)-containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.

摘要

描述了使用his3+基因作为选择标记构建用于粟酒裂殖酵母的载体。此外，我们报告了在含有his3(+)的穿梭载体中构建基因组文库，以通过补充His3活性缺陷菌株中的突变功能来促进基因克隆。

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