Weber-Arden J, Wilbert O M, Kabelitz D, Arden B
Department of Immunology, Paul Ehrlich Institute, Langen, Germany.
J Immunol Methods. 1996 Oct 16;197(1-2):187-92. doi: 10.1016/0022-1759(96)00151-2.
The technique of inverse PCR permits the rapid amplification and identification of unknown DNA segments adjacent to well characterized core regions. In the field of immunology anchored PCR and inverse PCR are useful methods for examining junctional diversity and unknown variable gene segments of rearranged T cell receptor genes. We have applied an improved inverse PCR protocol to study the repertoire of gamma delta T cell receptor genes in the developing thymus of the mouse.
反向PCR技术能够快速扩增和鉴定与特征明确的核心区域相邻的未知DNA片段。在免疫学领域，锚定PCR和反向PCR是用于研究重排的T细胞受体基因的连接多样性和未知可变基因片段的有用方法。我们应用了一种改进的反向PCR方案来研究小鼠发育中的胸腺中γδ T细胞受体基因的库。
