Subr Z, Kúdela O, Gallo J
Institute of Virology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Acta Virol. 1996 Jun;40(3):147-9.
Capsid proteins (CPs) of two strains of alfalfa mosaic virus (AIMV)-T6 and 425-were compared using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) peptide mapping and two-dimensional PAGE (2D-PAGE). The CPs had identical molecular mass but differed in their peptide pattern and charge. The AIMV strain T6 was isolated from lucerne in Czech Republic (Gallo, 1977). Previously, its morhology, symptomatology and RNA electrophoretic mobility were characterized and compared with those of the strain 425 (Hagedorn and Handson, 1963; Kúdela and Gallo, 1995). In this study we show that the CP composition of these two AIMV strains differs, too. AIMV, the unique member of the genus Alfamovirus, family Bromoviridae, is of economic significance in many countries in the world, particularly for its seed transmissibility and relative broad host range. Plenty of strains with different biological properties have been isolated (Van Regenmortel and Pinck, 1981). Some of them have been shown to differ in their RNA non-coding regions or in CP composition (Kraal, 1975; Kraal et al., 1976; Collot et al., Dore et al., 1989; Neeleman et al., 1991).
利用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）肽图分析和二维聚丙烯酰胺凝胶电泳（2D-PAGE）对两种苜蓿花叶病毒（AIMV）毒株——T6和425的衣壳蛋白（CP）进行了比较。这两种毒株的衣壳蛋白分子量相同，但肽图谱和电荷不同。AIMV毒株T6是从捷克共和国的紫花苜蓿中分离得到的（加洛，1977年）。此前，已对其形态学、症状学及RNA电泳迁移率进行了表征，并与毒株425进行了比较（哈格多恩和汉德森，1963年；库德拉和加洛，1995年）。在本研究中，我们发现这两种AIMV毒株的衣壳蛋白组成也存在差异。AIMV是雀麦花叶病毒科苜蓿病毒属的唯一成员，在世界上许多国家都具有经济重要性，尤其是因其种子传播性和相对广泛的宿主范围。已经分离出许多具有不同生物学特性的毒株（范·雷根莫特尔和平克，1981年）。其中一些毒株已被证明在RNA非编码区或衣壳蛋白组成方面存在差异（克拉尔，1975年；克拉尔等人，1976年；科洛等人、多尔等人，1989年；内勒曼等人，1991年）。
