大肠杆菌中质粒介导的甲醛抗性：抗性基因的特性
Plasmid-mediated formaldehyde resistance in Escherichia coli: characterization of resistance gene.
作者信息
Kümmerle N, Feucht H H, Kaulfers P M
机构信息
Institut für Medizinische Mikrobiologie und Immunologie, Universitäts-Krankenhaus Eppendorf, Hamburg, Germany.
出版信息
Antimicrob Agents Chemother. 1996 Oct;40(10):2276-9. doi: 10.1128/AAC.40.10.2276.
Abstract
The formaldehyde resistance mechanisms in the formaldehyde-resistant strain Escherichia coli VU3695 were investigated. A large (4.6-kb) plasmid DNA fragment encompassing the formaldehyde resistance gene was sequenced. A single 1,107-bp open reading frame encoding a glutathione- and NAD-dependent formaldehyde dehydrogenase was identified and sequenced, and the enzyme was expressed in an in vitro assay and purified. Amino acid sequence homology studies showed 62.4 to 63.2% identity with class III alcohol dehydrogenases isolated from horse, human, and rat livers. We demonstrated that the resistance mechanism in the formaldehyde-resistant strain E. coli VU3695 and in other formaldehyde-resistant members of the family Enterobacteriaceae is based on the enzymatic degradation of formaldehyde by a formaldehyde dehydrogenase.
摘要
对耐甲醛菌株大肠杆菌VU3695中的耐甲醛机制进行了研究。对包含耐甲醛基因的一个大的（4.6 kb）质粒DNA片段进行了测序。鉴定并测序了一个单一的1107 bp开放阅读框，其编码一种谷胱甘肽和NAD依赖的甲醛脱氢酶，并在体外试验中表达该酶并进行了纯化。氨基酸序列同源性研究表明，其与从马、人和大鼠肝脏中分离出的Ⅲ类醇脱氢酶具有62.4%至63.2%的同一性。我们证明，耐甲醛菌株大肠杆菌VU3695以及肠杆菌科其他耐甲醛成员中的耐药机制是基于甲醛脱氢酶对甲醛的酶促降解。
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