Alexiou-Daniel S, Papoutsi A, Papa A, Lambropoulos A, Antoniadis A
Department of Microbiology, School of Medicine, Aristotelian University of Thessaloniki, Greece.
Cell Mol Biol (Noisy-le-grand). 1996 Sep;42(6):833-8.
Genomic fingerprints from DNA of fifteen environmental and clinical strains of Legionella pneumophila serogroup 1, isolated from diverse geographic areas of Greece, during the period 1986 to 1994, were generated with arbitrarily-primed polymerase chain reaction (AP-PCR) in order to use the discriminatory power of two arbitrary primers, BG2 and M13 Forward to clarify the relationship among the fifteen isolates. Both primers were found to have the ability to discriminate strains of the same serogroup, to identify strains related to each other even though they were isolated at different times. Therefore, AP-PCR using BG2 or M13 Forward as primers, appears to be a useful tool which provides a fast and simple method for epidemiological fingerprinting.
1986年至1994年期间，从希腊不同地理区域分离出15株嗜肺军团菌血清1型环境菌株和临床菌株，利用任意引物聚合酶链反应（AP-PCR）生成基因组指纹，以利用两种任意引物BG2和M13正向引物的鉴别能力来阐明这15株分离株之间的关系。发现这两种引物都有能力区分同一血清群的菌株，即使它们是在不同时间分离的，也能识别彼此相关的菌株。因此，以BG2或M13正向引物进行AP-PCR似乎是一种有用的工具，它为流行病学指纹分析提供了一种快速简便的方法。
