Shahed A R, Werchan P M, Stavinoha W B
Operational Technologies Corporation, Armstrong Laboratory, Brooks AFB, TX, USA.
Methods Find Exp Clin Pharmacol. 1996 Jul-Aug;18(6):349-51.
Among the many rapidly metabolized compounds in the brain, acetylcholine is one of the most challenging to sample effectively due to its rapid synthesis, degradation and sequestration. To ascertain problems that invalidate sampling procedures two methods of tissue fixation, microwave heat inactivation and freeze fixation, were used for obtaining mice and rat brain samples, respectively. The data show that acetylcholine levels obtained by microwave fixation were much higher than those obtained by freeze fixation. Choline levels were not affected by the fixation method used. Microwave fixation results in more accurate assessment of acetylcholine levels than the freeze fixation method, even though the tissue fixation time was less than 1 s in both methods, because tissue integrity is maintained in the microwave fixation, but not during freeze fixation.
在大脑中众多快速代谢的化合物中，乙酰胆碱因其快速合成、降解和隔离的特性，成为有效取样最具挑战性的物质之一。为确定使取样程序无效的问题，分别采用两种组织固定方法，即微波热灭活和冷冻固定来获取小鼠和大鼠的脑样本。数据表明，通过微波固定获得的乙酰胆碱水平远高于冷冻固定获得的水平。胆碱水平不受所用固定方法的影响。尽管两种方法的组织固定时间均少于1秒，但微波固定比冷冻固定方法能更准确地评估乙酰胆碱水平，因为在微波固定过程中组织完整性得以保持，而冷冻固定过程中则不然。
