Yano O J, Baradarian R, Kim E S, Smith T J, Smith M L, Smith C R, Chiu D T
Department of Cardiothoracic Surgery, Columbia-Presbyterian Medical Center, New York, NY, USA.
Microsurgery. 1996;17(1):25-9. doi: 10.1002/(SICI)1098-2752(1996)17:1<25::AID-MICR5>3.0.CO;2-V.
With the use of microsurgery, we have developed a method of measuring hemodynamic parameters in a rat not possible with previous technology. Three groups of rats were studied: a chemically induced pulmonary hypertensive group (PH); a chemically induced pulmonary hypertensive group treated with single lung transplantation (LT); and an untreated, control group (C). Cardiac output, heart rate, and pulmonary vascular resistance were then calculated in each group from data obtained by 1 mm high fidelity micromanometers and an ultrasonic flow probe. The results show that the data collected from the rodent model are reproducible within each group, and data quality is comparable to large animal models. With this new method, data can be collected in a small animal model at a fraction of the time and cost of large animal studies. Additionally, the complications of graft rejection in large animal studies are eliminated in an isogenic rodent model.
通过使用显微外科技术，我们开发出了一种测量大鼠血流动力学参数的方法，而这是以往技术无法做到的。我们研究了三组大鼠：化学诱导性肺动脉高压组（PH）；接受单肺移植治疗的化学诱导性肺动脉高压组（LT）；以及未治疗的对照组（C）。然后根据通过1毫米高保真微测压计和超声流量探头获得的数据，计算每组的心输出量、心率和肺血管阻力。结果表明，从啮齿动物模型收集的数据在每组内具有可重复性，并且数据质量与大型动物模型相当。使用这种新方法，可以在小动物模型中收集数据，所需时间和成本仅为大型动物研究的一小部分。此外，在同基因啮齿动物模型中消除了大型动物研究中移植物排斥的并发症。
