Birago C, Pace T, Barca S, Picci L, Ponzi M
Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, Rome, Italy.
Mol Biochem Parasitol. 1996 Oct 1;80(2):193-202. doi: 10.1016/0166-6851(96)02680-1.
A single copy gene, pbB7, encoding a putative 26 kDa acidic protein has been isolated from Plasmodium berghei and appears to be part of a genomic region well conserved within the Plasmodium genus. The deduced amino acid sequence exhibits significant blocks of similarity with nucleosome assembly proteins from yeast and man. The nuclear localization of the natural protein and its close association with chromatin during the entire erythrocytic cycle of the parasite have been demonstrated using specific monoclonal antibodies against the pbB7 product expressed in Escherichia coli. These results suggest an involvement of this nuclear factor in the dynamics of chromatin packaging.
从伯氏疟原虫中分离出了一个单拷贝基因pbB7，它编码一种假定的26 kDa酸性蛋白，并且似乎是疟原虫属内一个保守性良好的基因组区域的一部分。推导的氨基酸序列与来自酵母和人类的核小体组装蛋白有显著的相似区域。利用针对在大肠杆菌中表达的pbB7产物的特异性单克隆抗体，已证明天然蛋白的核定位及其在寄生虫整个红细胞周期中与染色质的紧密关联。这些结果表明该核因子参与了染色质包装的动态过程。
