绵羊睾丸间质细胞胰岛素样肽mRNA的结构与表达

Structure and expression of Leydig insulin-like peptide mRNA in the sheep.

作者信息

Roche P J, Butkus A, Wintour E M, Tregear G

机构信息

Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, VIC, Australia.

出版信息

Mol Cell Endocrinol. 1996 Aug 9;121(2):171-7. doi: 10.1016/0303-7207(96)03861-0.

DOI:10.1016/0303-7207(96)03861-0

PMID:8892318

Abstract

We have used PCR to isolate and characterise Leydig insulin-like peptide (Ley I-L) mRNA from sheep ovary. The deduced amino acid sequence of sheep Ley I-L has good homology with the pig and human peptides, having 93% and 77% amino acid identity, respectively. Northern blot analysis revealed abundant expression in both ovary and testis. An examination of ovarian RNA from non-pregnant and pregnant sheep showed that pregnancy did not significantly increase Ley I-L mRNA levels. However mRNA levels did alter depending on whether ovaries contained a corpus luteum. Also ovaries were examined by hybridisation histochemistry to locate the site of expression. Abundant Ley I-L mRNA levels were found in the theca interna cells of the ovary.

摘要

我们利用聚合酶链反应（PCR）从绵羊卵巢中分离并鉴定了莱迪希胰岛素样肽（Ley I-L）的信使核糖核酸（mRNA）。绵羊Ley I-L推导的氨基酸序列与猪和人的肽具有良好的同源性，氨基酸同一性分别为93%和77%。Northern印迹分析显示在卵巢和睾丸中均有丰富表达。对未怀孕和怀孕绵羊的卵巢RNA进行检测表明，怀孕并未显著增加Ley I-L mRNA水平。然而，mRNA水平会根据卵巢是否含有黄体而改变。此外，通过杂交组织化学检查卵巢以定位表达位点。在卵巢的内膜细胞中发现了丰富的Ley I-L mRNA水平。