Myers R B, Srivastava S, Oelschlager D K, Brown D, Grizzle W E
Department of Pathology, University of Alabama at Birmingham, 35294-007, USA.
Hum Pathol. 1996 Oct;27(10):1021-4. doi: 10.1016/s0046-8177(96)90278-6.
The product of the nm23-H1 gene has been reported to be related to the metastatic potential of several tumors. Although several studies have characterized the expression of the nm23-H1 gene product in prostatic adenocarcinoma, little is known of the expression of this protein in prostatic intraepithelial neoplasia (PIN), a putative precancerous lesion. The authors used immunohistochemistry to examine the expression of the nm23-H1 protein in PIN as well as benign and malignant prostatic tissue. A monoclonal antibody to nm23-H1 (Novocastra, Newcastle upon Tyne, UK, clone 37.6) and a biotin/strepavidin detection system were used for antigen localization. Weak to moderate immunostaining was consistently detected in the benign glandular epithelium of 28 radical prostatectomy specimens. In contrast, strong immunostaining was detected in the glandular epithelium in PIN lesions of 19 radical prostatectomy specimens examined. Strong immunostaining was also frequently detected in the malignant cells of 39 localized prostatic adenocarcinomas, as well as 7 metastatic lesions. These findings show a phenotypic similarity of PIN to prostatic adenocarcinoma with respect to nm23-H1 expression. Furthermore, strong expression of nm23-H1 likely represents an early event in the development of prostatic adenocarcinoma.
据报道，nm23-H1基因的产物与多种肿瘤的转移潜能有关。尽管多项研究已对nm23-H1基因产物在前列腺腺癌中的表达进行了特征描述，但对于该蛋白在前列腺上皮内瘤变（PIN）这一假定的癌前病变中的表达情况却知之甚少。作者采用免疫组织化学方法检测了nm23-H1蛋白在PIN以及良性和恶性前列腺组织中的表达。使用针对nm23-H1的单克隆抗体（英国泰恩河畔纽卡斯尔的诺沃卡斯尔公司，克隆号37.6）和生物素/链霉抗生物素蛋白检测系统进行抗原定位。在28例根治性前列腺切除术标本的良性腺上皮中始终检测到弱阳性至中度免疫染色。相比之下，在19例接受检查的根治性前列腺切除术标本的PIN病变的腺上皮中检测到强免疫染色。在39例局限性前列腺腺癌的恶性细胞以及7例转移灶中也经常检测到强免疫染色。这些发现表明，在nm23-H1表达方面，PIN与前列腺腺癌存在表型相似性。此外，nm23-H1的强表达可能代表前列腺腺癌发生过程中的早期事件。
