Ibbotson R E, Chapman R M, Corcoran M M, Oscier D G
Molecular Biology, Pathology Department, Bournemouth General Hospital, UK.
Leukemia. 1996 Nov;10(11):1712-4.
Evidence that the D13S25 locus lies close to a potential tumour suppressor gene implicated in the pathogenesis of B-CLL has been based on detection of LOH and bi-allelic loss using the pH2-42 probe. The SspI polymorphism detected by this probe has been identified by sequencing adjacent clones and a polymorphic (TA)n repeat has been found. Amplification of the region encompassing both polymorphic markers by PCR increases the informativity to 80%.
有证据表明，D13S25基因座靠近一个可能与B细胞慢性淋巴细胞白血病发病机制相关的潜在肿瘤抑制基因，这一证据基于使用pH2 - 42探针检测杂合性缺失（LOH）和双等位基因缺失。通过对相邻克隆进行测序鉴定了该探针检测到的SspI多态性，并发现了一个多态性（TA）n重复序列。通过聚合酶链反应（PCR）扩增包含这两个多态性标记的区域，可将信息性提高到80%。
