Burkin D J, Jones C, Burkin H R, McGrew J A, Broad T E
Eleanor Roosevelt Institute, Denver, CO 80206, USA.
Cytogenet Cell Genet. 1996;74(1-2):86-9. doi: 10.1159/000134388.
Sheep CENPB and CENPC clones were isolated from a lung cDNA library. The DNA and predicted amino acid sequences of these clones were compared with their human and mouse homologs and shown to contain a high degree of sequence similarity. Sheep chromosomal assignments were made using a sheep x hamster somatic cell hybrid mini-panel. CENPB was assigned to sheep chromosome 13 and CENPC to chromosome 6. The previously reported assignments of CENPB and CENPC to human chromosomes 20 and 4, respectively, suggest conserved synteny between sheep chromosome 13 and human chromosome 20 and support conserved synteny between sheep chromosome 6 and human chromosome 4.
绵羊CENPB和CENPC克隆是从肺cDNA文库中分离出来的。将这些克隆的DNA和预测的氨基酸序列与其人类和小鼠同源物进行比较，结果显示它们具有高度的序列相似性。使用绵羊×仓鼠体细胞杂交微型板对绵羊进行染色体定位。CENPB被定位到绵羊13号染色体，CENPC被定位到6号染色体。先前报道的CENPB和CENPC分别在人类20号和4号染色体上的定位，表明绵羊13号染色体和人类20号染色体之间存在保守的同线性，并支持绵羊6号染色体和人类4号染色体之间的保守同线性。
