Ek S, Westgren M, Markling L, Kjeldgaard A, Ringdén O
Department of Obstetrics and Gynecology, Huddinge Hospital, Karolinska Institute, Sweden.
Fetal Diagn Ther. 1996 Sep-Oct;11(5):318-25. doi: 10.1159/000264333.
The effects of interleukins 3 and 6, stem cell factor, and granulocyte-macrophage colony-stimulating factor on human fetal hematopoietic, bone marrow, and cord blood cells were studied on the basis of the colony-forming capacity. Fetal hematopoietic cells from 28 elective abortions, three bone marrow samples, and three cord blond samples were incubated with cytokines and investigated for the presence of BFU-E (burst-forming units--erythroid), CFU-GM (colony-forming units--granulocytes, macrophages), and CFU-GEMM (colony-forming units--granulocytes, erythrocytes, macrophages, megakaryocytes). Single and combined cytokines and preincubation versus adding cytokines in culture were investigated. Interleukin-6 alone had the most pronounced effect on BFU-E formation. All four cytokines in combination yielded the highest scores for CFU-GM (p < 0.05) and CFU-GEMM (p < 0.05), whereas BFU-E was not enhanced. The mode of cytokine exposure was not a determinant of colony formation.
基于集落形成能力，研究了白细胞介素3和6、干细胞因子以及粒细胞-巨噬细胞集落刺激因子对人胎儿造血细胞、骨髓细胞和脐血细胞的影响。将来自28例选择性流产的胎儿造血细胞、3份骨髓样本和3份脐血样本与细胞因子一起孵育，并检测其中红系爆式集落形成单位（BFU-E）、粒-巨噬细胞集落形成单位（CFU-GM）和粒-红-巨噬-巨核细胞集落形成单位（CFU-GEMM）的存在情况。研究了单一和联合细胞因子以及预孵育与在培养中添加细胞因子的情况。单独的白细胞介素-6对BFU-E形成的影响最为显著。四种细胞因子联合使用时，CFU-GM（p < 0.05）和CFU-GEMM（p < 0.05）得分最高，而BFU-E没有增强。细胞因子暴露方式不是集落形成的决定因素。
