Takahashi N, Takeuchi K, Abe T, Sugawara A, Abe K
Second Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Endocrinology. 1996 Nov;137(11):5170-3. doi: 10.1210/endo.137.11.8895394.
Thromboxane (TX) is a vasoactive hormone which is known to be involved in renal physiology and pathophysiology. To identify the site of action of TX in the kidney, we examined the distribution of this receptor in the normal rat kidney using a polyclonal antibody against TX receptor we had raised. Western immunoblot of rat kidney membrane fractions with the antibody identified a single protein band at the predicted molecular mass of rat TX receptor protein. In the rat kidney, immunostainable TX receptor was observed in glomeruli, arterial walls, luminal membranes of thick ascending limbs of Henle's loop, the luminal and basolateral membranes of either distal convoluted tubules or connecting tubules, and the basolateral membranes of collecting tubules. The localization of TX receptor provides better understanding of the mechanism of previously reported effects of TX on glomerular and tubular functions leading to hypertension as well as renal parenchymal diseases.
血栓素（TX）是一种血管活性激素，已知其参与肾脏生理和病理生理过程。为了确定TX在肾脏中的作用位点，我们使用针对我们所制备的TX受体的多克隆抗体，检测了该受体在正常大鼠肾脏中的分布。用该抗体对大鼠肾脏膜组分进行蛋白质免疫印迹分析，在大鼠TX受体蛋白的预测分子量处鉴定出一条单一蛋白条带。在大鼠肾脏中，在肾小球、动脉壁、亨氏袢厚升支的管腔膜、远曲小管或连接小管的管腔和基底外侧膜以及集合小管的基底外侧膜中观察到可免疫染色的TX受体。TX受体的定位有助于更好地理解先前报道的TX对肾小球和肾小管功能的影响机制，这些影响会导致高血压以及肾实质疾病。
