含有LCR、（A）γ、δ和β珠蛋白基因的黏粒在小鼠红白血病细胞中的表达。

Expression of a cosmid containing the LCR, (A)gamma, delta, and beta globin genes in mouse erythroleukemia cells.

作者信息

Flamm M, Bornschlegel K, Wu S, O'Neill D, Bank A

机构信息

Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York, USA.

出版信息

Am J Hematol. 1996 Nov;53(3):181-7. doi: 10.1002/(SICI)1096-8652(199611)53:3<181::AID-AJH6>3.0.CO;2-V.

DOI:10.1002/(SICI)1096-8652(199611)53:3<181::AID-AJH6>3.0.CO;2-V

PMID:8895689

Abstract

A novel method involving the packaging of phage particles was used to introduce cosmids containing components of the human beta-globin locus control region (LCR) and the human (A)gamma, delta, and beta globin genes into mouse erythroleukemia (MEL) cells. After stable transfection, MEL clones were selected and analyzed for expression of human genes. Both (A)gamma and beta globin mRNA were expressed in these clones, indicating that MEL cells do not suppress transcription of the human gamma globin gene. The variability of human (A)gamma vs. beta globin expression from clone to clone prevents a clear delineation of differences in the expression of these two genes with a cosmid in which a region potentially involved in gamma-to-beta switching has been deleted. The results suggest that MEL cells are capable of supporting human gamma expression, despite their predominantly adult phenotype.

摘要

一种涉及噬菌体颗粒包装的新方法被用于将含有人类β-珠蛋白基因座控制区（LCR）组件以及人类（A）γ、δ和β珠蛋白基因的黏粒导入小鼠红白血病（MEL）细胞。稳定转染后，挑选出MEL克隆并分析人类基因的表达情况。在这些克隆中，（A）γ和β珠蛋白mRNA均有表达，这表明MEL细胞不会抑制人类γ珠蛋白基因的转录。不同克隆间人类（A）γ与β珠蛋白表达的变异性使得无法明确界定这两个基因在一个缺失了可能参与γ向β转换区域的黏粒中的表达差异。结果表明，尽管MEL细胞主要呈现成年表型，但它们能够支持人类γ珠蛋白的表达。