大鼠肝脏葡萄糖激酶“记忆性”机制的动力学证据。
Kinetic evidence for a 'mnemonical' mechanism for rat liver glucokinase.
作者信息
Storer A C, Cornish-Bowden A
出版信息
Biochem J. 1977 Jul 1;165(1):61-9. doi: 10.1042/bj1650061.
Abstract
Inhibition studies of glucokinase were carried out with the products of the reaction, glucose 6-phosphate and MgADP-, as well as with ADP3-, Mg2+ and ATP4-. The results of these, together with those of kinetic studies of the uninhibited reaction described previously [Storer & Cornish-Bowden (1976) Biochem. J. 159, 7-14], indicate that the enzyme obeys a 'mnemonical' mechanism. This implies that the co-operativity observed with glucose as substrate arises because glucose binds differentially to two forms of the free enzyme that are not in equilibrium under steady-state conditions. The mechanism predicts the decrease in glucose co-operativity observed at low concentrations of MgATP2-. The product-inhibition results suggest that glucose 6-phosphate is released first and that it is possibly displaced by MgATP2- in a concerted reaction.
摘要
用反应产物6-磷酸葡萄糖和MgADP⁻以及ADP³⁻、Mg²⁺和ATP⁴⁻对葡萄糖激酶进行了抑制研究。这些研究结果，连同先前描述的无抑制反应的动力学研究结果[斯托勒和科尼什-鲍登（1976年）《生物化学杂志》159, 7 - 14]表明，该酶遵循一种“记忆”机制。这意味着观察到以葡萄糖为底物时的协同性，是因为葡萄糖与两种游离酶形式的结合存在差异，而这两种形式在稳态条件下并非处于平衡状态。该机制预测了在低浓度MgATP²⁻时观察到的葡萄糖协同性降低。产物抑制结果表明，6-磷酸葡萄糖首先释放，并且它可能在协同反应中被MgATP²⁻取代。
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本文引用的文献
1
A submicrodetermination of glucose.葡萄糖的亚微量测定
J Biol Chem. 1949 Nov;181(1):149-51.
2
ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL.关于别构转变的本质：一个合理的模型。
J Mol Biol. 1965 May;12:88-118. doi: 10.1016/s0022-2836(65)80285-6.
3
Kinetic formulations for enzymic reactions involving two substrates.涉及两种底物的酶促反应的动力学公式。
Can J Biochem Physiol. 1962 Jun;40:763-804.
4
Ultraviolet absorption spectra of adenosine-5'-triphosphate and related 5'-ribonucleotides.腺苷 - 5'-三磷酸及相关5'-核糖核苷酸的紫外吸收光谱。
Arch Biochem Biophys. 1956 Jun;62(2):253-64. doi: 10.1016/0003-9861(56)90123-0.
5
The determination of enzyme inhibitor constants.酶抑制剂常数的测定
Biochem J. 1953 Aug;55(1):170-1. doi: 10.1042/bj0550170.
6
Treatment of enzyme kinetic data. II. The multisite case: comparison of allosteric models and a possible new mechanism.酶动力学数据的处理。II. 多位点情况：别构模型的比较及一种可能的新机制。
J Biol Chem. 1967 Sep 25;242(18):4045-52.
7
A theoretical study of the binding of small molecules to a polymerizing protein system. A model for allosteric effects.小分子与聚合蛋白系统结合的理论研究。变构效应模型。
Biochemistry. 1967 Aug;6(8):2449-56. doi: 10.1021/bi00860a022.
8
Thermodynamic studies of the formation and ionization of the magnesium(II) complexes of ADP and ATP over the pH range 5 to 9.在pH值5至9范围内对ADP和ATP的镁（II）配合物的形成和电离进行的热力学研究。
J Am Chem Soc. 1966 Jun 20;88(12):2631-40. doi: 10.1021/ja00964a002.
9
Knetic implications of enzyme-effector complexes.酶-效应物复合物的动力学意义。
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