Farah M F, One M, Novello J C, Toyama M H, Perales J, Moussatché H, Domont G B, Oliveira B, Marangoni S
Departamento de Bioquímica, Universidade Estadual de Campinas, SP, Brazil.
Toxicon. 1996 Sep;34(9):1067-71. doi: 10.1016/0041-0101(96)00034-7.
The fractionation of Didelphis albiventris serum by DEAE-Sephadex A50 yields a fraction (DA2) which protects the opossum against Bothrops venom. One polypeptide (DA2-II) responsible for this protection was isolated from fraction DA2 by ion exchange chromatography and biochemically characterized. DA2-II is a 43,000 mol. wt glycoprotein with the following N-terminal sequence: LKAMDTTPPLKIKKEPVK. Pairwise comparison of the amino acid sequence with four anti-hemorrhagic factors isolated from other opossum species indicated that DA2-II possesses high similarity (60-80%) with these proteins.
