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对酿酒酵母十五号染色体左臂上一个10624碱基对片段进行的DNA序列分析,揭示出一种RNA结合蛋白、一种线粒体蛋白、两种核糖体蛋白以及两个新的开放阅读框。

DNA sequence analysis of a 10 624 bp fragment of the left arm of chromosome XV from Saccharomyces cerevisiae reveals a RNA binding protein, a mitochondrial protein, two ribosomal proteins and two new open reading frames.

作者信息

Lafuente M J, Gamo F J, Gancedo C

机构信息

Instituto de Investigaciones Biomédicas, C.S.I.C. Unidad de Bioquimica y Genética de Levaduras, Madrid, Spain.

出版信息

Yeast. 1996 Sep;12(10B Suppl):1041-5. doi: 10.1002/(SICI)1097-0061(199609)12:10B%3C1041::AID-YEA989%3E3.0.CO;2-I.

Abstract

We have determined the sequence of a 10624 bp DNA segment located in the left arm of chromosome XV of Saccharomyces cerevisiae. The sequence contains eight open reading frames (ORFs) longer than 100 amino acids. Two of them do not present significant homology with sequences found in the databases. The product of ORF o0553 is identical to the protein encoded by the gene SMF1. Internal to it there is another ORF, o0555 that is apparently expressed. The proteins encoded by ORFs o0559 and o0565 are identical to ribosomal proteins S19.e and L18 respectively. ORF o0550 encodes a protein with an RNA binding signature including RNP motifs and stretches rich in asparagine, glutamine and arginine.

摘要

我们已经确定了位于酿酒酵母第十五号染色体左臂上一段10624 bp的DNA片段的序列。该序列包含八个长度超过100个氨基酸的开放阅读框(ORF)。其中两个与数据库中发现的序列没有显著同源性。ORF o0553的产物与SMF1基因编码的蛋白质相同。在其内部还有另一个ORF,o0555,显然是表达的。ORF o0559和o0565编码的蛋白质分别与核糖体蛋白S19.e和L18相同。ORF o0550编码一种具有RNA结合特征的蛋白质,包括RNP基序以及富含天冬酰胺、谷氨酰胺和精氨酸的区域。

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