Gamo F J, Lafuente M J, Casamayor A, Ariño J, Aldea M, Casas C, Herrero E, Gancedo C
Instituto de Investigaciones Biomédicas, CSIC Unidad de Bioquímica y Genética de Levaduras, Madrid, Spain.
Yeast. 1996 Jun 15;12(7):709-14. doi: 10.1002/(SICI)1097-0061(19960615)12:7%3C709::AID-YEA957%3E3.0.CO;2-1.
We report the sequence of a 15.5 kb DNA segment located near the left telomere of chromosome XV of Saccharomyces cerevisiae. The sequence contains nine open reading frames (ORFs) longer than 300 bp. Three of them are internal to other ones. One corresponds to the gene LGT3 that encodes a putative sugar transporter. Three adjacent ORFs were separated by two stop codons in frame. These ORFs presented homology with the gene CPS1 that encodes carboxypeptidase S. The stop codons were not found in the same sequence derived from another yeast strain. Two other ORFs without significant homology in databases were also found. One of them, O0420, is very rich in serine and threonine and presents a series of repeated or similar amino acid stretches along the sequence.
我们报道了位于酿酒酵母第十五号染色体左端粒附近的一段15.5 kb DNA片段的序列。该序列包含9个长度超过300 bp的开放阅读框（ORF）。其中3个位于其他阅读框内部。一个对应于编码推定糖转运蛋白的LGT3基因。三个相邻的ORF被两个框内终止密码子隔开。这些ORF与编码羧肽酶S的CPS1基因具有同源性。在源自另一种酵母菌株的相同序列中未发现终止密码子。还发现了另外两个在数据库中无明显同源性的ORF。其中一个，O0420，富含丝氨酸和苏氨酸，并且在整个序列中呈现出一系列重复或相似的氨基酸序列。
