Asada-Kubota M, Kobayashi M, Ueda T, Shimada M, Takeda K, Hamada K, Maekawa S, Sokawa Y
Department of Biotechnology, Kyoto Institute of Technology, Japan.
Anat Embryol (Berl). 1996 Oct;194(4):349-54. doi: 10.1007/BF00198536.
Although 2', 5'-oligoadenylate synthetase (2-5AS) is an enzyme induced by inferferon (IFN) or viral infections and mediates one of the principal antiviral pathways turned on by IFN, low constitutive levels of the enzyme can be detected in various "normal" animals that have not been treated with IFN or virus. The distribution of this enzyme in the female and male reproductive organs of normal healthy mice was studied by Western blotting and by an immunohistochemical method, using a specific monoclonal antibody. On Western blotting, an antibody to 42-kD 2-5AS reacted with extracts from the ovary, oviduct, uterus, vagina, and placenta among the female reproductive organs, and testis, epididymis, and ductus deferens in the male. Immunohistochemically, the 2-5AS was localized on the following cells in the female reproductive organs: oocytes in the ovary; epithelium in the oviduct, uterus, and vagina; and trophoblasts in the placenta. Furthermore, the 2-5AS was localized on the epithelium and muscular layer in the ductus deferens and epithelium in the penis of the male mice, whereas the epithelium of the testis, epididymis, and seminal vesicle were stained faintly. It is well known that IFN is produced continuously in normal mice, so the 2-5AS in the tissues of normal mice is considered to be induced by such IFN produced under physiological conditions. Expression of the 2-5AS on the epithelium and trophoblasts in the reproductive organs may be responsible for the prevention of viral infections. However, the enzyme in oocytes may have some functions other than as an antiviral agent, since the enzyme was not detectable in embryos during early development.
尽管2',5'-寡腺苷酸合成酶（2-5AS）是一种由干扰素（IFN）或病毒感染诱导产生的酶，介导IFN开启的主要抗病毒途径之一，但在未接受IFN或病毒处理的各种“正常”动物中可检测到该酶的低组成水平。使用特异性单克隆抗体，通过蛋白质免疫印迹法和免疫组织化学方法研究了这种酶在正常健康小鼠的雌性和雄性生殖器官中的分布。在蛋白质免疫印迹中，针对42-kD 2-5AS的抗体与雌性生殖器官中的卵巢、输卵管、子宫、阴道和胎盘以及雄性中的睾丸、附睾和输精管的提取物发生反应。免疫组织化学显示，2-5AS定位于雌性生殖器官的以下细胞：卵巢中的卵母细胞；输卵管、子宫和阴道中的上皮细胞；以及胎盘中的滋养层细胞。此外，2-5AS定位于雄性小鼠输精管的上皮和肌层以及阴茎的上皮，而睾丸、附睾和精囊的上皮染色较浅。众所周知，正常小鼠中持续产生IFN，因此正常小鼠组织中的2-5AS被认为是由生理条件下产生的此类IFN诱导的。生殖器官上皮和滋养层细胞上2-5AS的表达可能负责预防病毒感染。然而，卵母细胞中的这种酶可能具有除作为抗病毒剂之外的一些功能，因为在早期发育的胚胎中未检测到该酶。
