使用依米丁和放线菌素D生产神经元杂种的改进方法。
Improved method for producing neuronal hybrids using emetine and actinomycin D.
作者信息
Hikawa N, Takenaka T
机构信息
Department of Physiology, School of Medicine Yokohama City University, Japan.
出版信息
Brain Res. 1996 Sep 23;734(1-2):345-8.
PMID:8896846
Abstract
We modified the method of somatic cell fusion for neurons to improve the efficiency of hybrid production. C1300 neuroblastoma cells were incubated with emetine and actinomycin D before fusion. After fusion between C1300 cells and adult mouse dorsal root ganglia neurons with polyethyleneglycol, we were able to select the hybrids from non-fused cells. We obtained hybrid clones at high efficiency (7.2 clones/10(4) neurons).
摘要
我们改进了神经元体细胞融合的方法，以提高杂交体产生的效率。在融合前，将C1300神经母细胞瘤细胞与依米丁和放线菌素D一起孵育。在用聚乙二醇使C1300细胞与成年小鼠背根神经节神经元融合后，我们能够从不融合的细胞中筛选出杂交体。我们高效地获得了杂交克隆（7.2个克隆/10⁴个神经元）。
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