Koyama Y, Norose K, Kusubata M, Irie S, Kusakabe M
Division of Experimental Animal Research, Tsukuba Life Science Center, Institute of Physical and Chemical Research, Ibaraki, Japan.
Histochem Cell Biol. 1996 Sep;106(3):263-73. doi: 10.1007/BF02473236.
Tenascin is a large extracellular matrix glycoprotein which is found in limited regions of normal adult tissues including the skin. We investigated the induction of tenascin expression in mouse skin during hapten-induced dermatitis. In the dorsal skin, hapten application first induced a transient expression of tenascin in deeper regions of the skin. Its distribution then spread over the whole dermis corresponding to the infiltration of Mac-2-positive macrophages. In the ear, tenascin was consistently found in the subcutaneous tissue on the inner side, but very little was seen on the outer side. Tenascin did appear transiently, however, on both sides under hapten treatment. In the early phase of allergic contact dermatitis, no apparent induction of tenascin expression was observed in the swollen ear. However, there was an abundant tenascin expression on both sides during healing. Tenascin expressed under normal conditions was mostly the 180-kDa isoform, while the 230-kDa isoform was markedly induced during healing of the dermatitis. These results suggest that tenascin, particularly the larger 230-kDa isoform, may play important roles in the pathogenesis and healing of hapten-induced dermatitis.
腱生蛋白是一种大型细胞外基质糖蛋白，存在于包括皮肤在内的正常成年组织的有限区域。我们研究了在半抗原诱导的皮炎过程中小鼠皮肤中腱生蛋白表达的诱导情况。在背部皮肤中，涂抹半抗原首先诱导腱生蛋白在皮肤深层区域短暂表达。其分布随后扩展至整个真皮，与Mac-2阳性巨噬细胞的浸润相对应。在耳部，内侧皮下组织中始终能发现腱生蛋白，但外侧则很少见。然而，在半抗原处理下，两侧均出现了腱生蛋白的短暂表达。在过敏性接触性皮炎的早期阶段，肿胀的耳部未观察到腱生蛋白表达的明显诱导。然而，在愈合过程中两侧均有丰富的腱生蛋白表达。正常条件下表达的腱生蛋白大多是180 kDa的异构体，而在皮炎愈合过程中230 kDa的异构体被显著诱导。这些结果表明，腱生蛋白，尤其是较大的230 kDa异构体，可能在半抗原诱导的皮炎的发病机制和愈合过程中发挥重要作用。
