Laboratory of Veterinary Pharmacology, Division of Veterinary Science, Osaka Prefecture University Graduate School of Life and Environmental Science.
Laboratory of Molecular Toxicology, Department of Pharmaceutical Sciences, Ritsumeikan University.
Biol Pharm Bull. 2018;41(2):182-189. doi: 10.1248/bpb.b17-00594.
Interleukin (IL)-19 is a member of the IL-10 family of interleukins and is an immuno-modulatory cytokine produced by the main macrophages. The gastrointestinal tissues of IL-19 knockout mice show exacerbated experimental colitis mediated by the innate immune system and T cells. There is an increasing focus on the interaction and relationship of IL-19 with the function of T cells. Contact hypersensitivity (CHS) is T cell-mediated cutaneous inflammation. Therefore, we asked whether IL-19 causes CHS. We investigated the immunological role of IL-19 in CHS induced by 1-fluoro-2,4-dinitrofluorobenzene as a hapten. IL-19 was highly expressed in skin exposed to the hapten, and ear swelling was increased in IL-19 knockout mice. The exacerbation of the CHS response in IL-19 knockout mice correlated with increased levels of IL-17 and IL-6, but no alterations were noted in the production of interferon (IFN)γ and IL-4 in the T cells of the lymph nodes. In addition to the effect on T cell response, IL-19 knockout mice increased production of inflammatory cytokines. These results show that IL-19 suppressed hapten-dependent skin inflammation in the elicitation phase of CHS.
白细胞介素 (IL)-19 是白细胞介素 10 家族的成员，是主要巨噬细胞产生的免疫调节细胞因子。IL-19 敲除小鼠的胃肠道组织显示先天免疫系统和 T 细胞介导的实验性结肠炎加重。人们越来越关注 IL-19 与 T 细胞功能的相互作用和关系。接触性超敏反应 (CHS) 是 T 细胞介导的皮肤炎症。因此，我们想知道 IL-19 是否会引起 CHS。我们研究了 IL-19 在作为半抗原的 1-氟-2,4-二硝基氟苯诱导的 CHS 中的免疫作用。IL-19 在接触半抗原的皮肤中高度表达，并且在 IL-19 敲除小鼠中耳朵肿胀增加。IL-19 敲除小鼠 CHS 反应的加重与 IL-17 和 IL-6 水平的升高相关，但在淋巴结 T 细胞中未观察到干扰素 (IFN)γ 和 IL-4 的产生发生改变。除了对 T 细胞反应的影响外，IL-19 敲除小鼠还增加了炎症细胞因子的产生。这些结果表明，IL-19 抑制了 CHS 激发阶段依赖半抗原的皮肤炎症。
