Blanco J E, Blanco M, Mora A, Prado C, Río M, Fernández L, Fernández M J, Sáinz V, Blanco J
Departamento de Microbioloxía e Parasitoloxía, Facultade de Veterinaria, Universidade de Santiago de Compostela, Lugo, España.
Microbiologia. 1996 Sep;12(3):385-94.
Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 has been recently recognized as a human pathogen associated with haemorrhagic colitis and haemolytic uraemic syndrome. Most outbreaks of haemorrhagic colitis resulted from the consumption of undercooked minced beef or raw milk. Dairy cattle have been identified as a reservoir of EHEC O157: H7. In this study E. coli O157 specific antibody, coated on magnetic beads, was used to concentrate and release EHEC O157:H7 from meat samples. A survey of retail fresh minced beef and hamburger samples using this procedure revealed that 3 (5%) of 58 beef samples were positive for EHEC O157:H7. Two of the strains produced both VT1 and VT2 verotoxins, and one produced only VT2. Immunomagnetic separation is a sensitive and simple technique for the isolation of E. coli O157 from food, and could be useful for a further elucidation of the epidemiology of this organism. The relatively high prevalence of EHEC O157:H7 in beef samples may constitute a risk for public health. Thus, a suitable epidemiologic control and effective methods of prevention should be applied.
肠出血性大肠杆菌（EHEC）O157:H7最近被确认为一种与出血性结肠炎和溶血尿毒综合征相关的人类病原体。大多数出血性结肠炎疫情是由食用未煮熟的碎牛肉或生牛奶引起的。奶牛已被确定为EHEC O157:H7的宿主。在本研究中，涂覆在磁珠上的大肠杆菌O157特异性抗体被用于从肉类样本中浓缩和释放EHEC O157:H7。使用该程序对零售新鲜碎牛肉和汉堡样本进行的一项调查显示，58份牛肉样本中有3份（5%）EHEC O157:H7呈阳性。其中两株菌株同时产生VT1和VT2毒素，一株仅产生VT2。免疫磁分离是从食品中分离大肠杆菌O157的一种灵敏且简单的技术，可能有助于进一步阐明该病原体的流行病学。牛肉样本中EHEC O157:H7的相对高流行率可能对公众健康构成风险。因此，应实施适当的流行病学控制和有效的预防方法。
