Sánchez J M, Rodríguez F, Valle L, Muñoz M A, Moriñigo M A, Borrego J J
Departamento de Microbiología, Fucultad de Ciencias, Universidad de Málaga, España.
Microbiologia. 1996 Sep;12(3):425-34.
The addition of support materials, such as sepiolite, to fluidized-bed anaerobic digesters enhances the methane production by increasing the colonization by syntrophic microbiota. However, the efficiency in the methanogenesis depends on the particle size of the support material, the highest level of methane production being obtained by the smaller particle size sepiolite. Because of the porosity and physico-chemical characteristics of these support materials, the anaerobic microbial consortia formed quickly (after one week of incubation). The predominant methanogenic bacteria present in the active granules, detected both by immunofluorescence using specific antibodies and by scanning electron microscopy, were acetoclastic methanogens, mainly Methanosarcina and Methanosaeta.
向流化床厌氧消化器中添加诸如海泡石之类的支撑材料，可通过增加互营微生物群的定殖来提高甲烷产量。然而，甲烷生成的效率取决于支撑材料的粒径，较小粒径的海泡石可实现最高水平的甲烷产量。由于这些支撑材料的孔隙率和物理化学特性，厌氧微生物群落形成迅速（培养一周后）。通过使用特异性抗体的免疫荧光法和扫描电子显微镜检测发现，活性颗粒中存在的主要产甲烷细菌是乙酸裂解产甲烷菌，主要为甲烷八叠球菌属和甲烷丝状菌属。
