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1
Crystallization and preliminary X-ray diffraction studies of alpha-amylase from the antarctic psychrophile Alteromonas haloplanctis A23.来自南极嗜冷菌嗜盐浮游交替单胞菌A23的α-淀粉酶的结晶及初步X射线衍射研究。
Protein Sci. 1996 Oct;5(10):2128-9. doi: 10.1002/pro.5560051021.
2
Stability and structural analysis of alpha-amylase from the antarctic psychrophile Alteromonas haloplanctis A23.南极嗜冷菌嗜盐浮游交替单胞菌A23来源的α-淀粉酶的稳定性及结构分析
Eur J Biochem. 1994 Jun 1;222(2):441-7. doi: 10.1111/j.1432-1033.1994.tb18883.x.
3
Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor.来自嗜盐浮游交替单胞菌的嗜冷α-淀粉酶的天然形式及其与抑制剂复合物的晶体结构。
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4
Purification, characterization, and nucleotide sequence of the thermolabile alpha-amylase from the antarctic psychrotroph Alteromonas haloplanctis A23.来自南极嗜冷菌嗜盐栖 Alteromonas haloplanctis A23 的热不稳定α-淀粉酶的纯化、特性鉴定及核苷酸序列分析
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5
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6
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8
Structures of the psychrophilic Alteromonas haloplanctis alpha-amylase give insights into cold adaptation at a molecular level.嗜冷性嗜盐浮游单胞菌α-淀粉酶的结构在分子水平上为冷适应提供了见解。
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8
Comparative proteome analysis of psychrophilic versus mesophilic bacterial species: Insights into the molecular basis of cold adaptation of proteins.嗜冷菌与嗜温菌的比较蛋白质组分析：对蛋白质冷适应分子基础的见解
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10
Crystal structures of the psychrophilic alpha-amylase from Alteromonas haloplanctis in its native form and complexed with an inhibitor.来自嗜盐浮游交替单胞菌的嗜冷α-淀粉酶的天然形式及其与抑制剂复合物的晶体结构。
Protein Sci. 1998 Mar;7(3):564-72. doi: 10.1002/pro.5560070304.

本文引用的文献

1
The structure of human pancreatic alpha-amylase at 1.8 A resolution and comparisons with related enzymes.分辨率为1.8埃的人胰腺α-淀粉酶结构及其与相关酶的比较。
Protein Sci. 1995 Sep;4(9):1730-42. doi: 10.1002/pro.5560040908.
2
Structure and molecular model refinement of pig pancreatic alpha-amylase at 2.1 A resolution.猪胰α-淀粉酶在2.1埃分辨率下的结构与分子模型优化
J Mol Biol. 1993 Jun 5;231(3):785-99. doi: 10.1006/jmbi.1993.1326.
3
Stability and structural analysis of alpha-amylase from the antarctic psychrophile Alteromonas haloplanctis A23.南极嗜冷菌嗜盐浮游交替单胞菌A23来源的α-淀粉酶的稳定性及结构分析
Eur J Biochem. 1994 Jun 1;222(2):441-7. doi: 10.1111/j.1432-1033.1994.tb18883.x.
4
Solvent content of protein crystals.蛋白质晶体的溶剂含量。
J Mol Biol. 1968 Apr 28;33(2):491-7. doi: 10.1016/0022-2836(68)90205-2.
5
Theory of protein solubility.蛋白质溶解度理论
Methods Enzymol. 1985;114:49-77. doi: 10.1016/0076-6879(85)14005-x.
6
Why preferential hydration does not always stabilize the native structure of globular proteins.为何优先水合作用并不总能稳定球状蛋白质的天然结构。
Biochemistry. 1990 Feb 20;29(7):1924-31. doi: 10.1021/bi00459a037.
7
Purification, characterization, and nucleotide sequence of the thermolabile alpha-amylase from the antarctic psychrotroph Alteromonas haloplanctis A23.来自南极嗜冷菌嗜盐栖 Alteromonas haloplanctis A23 的热不稳定α-淀粉酶的纯化、特性鉴定及核苷酸序列分析
J Biol Chem. 1992 Mar 15;267(8):5217-21.

来自南极嗜冷菌嗜盐浮游交替单胞菌A23的α-淀粉酶的结晶及初步X射线衍射研究。

Crystallization and preliminary X-ray diffraction studies of alpha-amylase from the antarctic psychrophile Alteromonas haloplanctis A23.

作者信息

Aghajari N, Feller G, Gerday C, Haser R

机构信息

Laboratoire d'Architecture et Fonction des Macromolécules Biologiques, UPR9039, Institut de Biologie Structurale et Microbiologie, IFRI, CNRS, Marseille, France.

出版信息

Protein Sci. 1996 Oct;5(10):2128-9. doi: 10.1002/pro.5560051021.

DOI:10.1002/pro.5560051021
PMID:8897615
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2143274/
Abstract

A cold-active alpha-amylase was purified from culture supernatants of the antarctic psychrophile Alteromonas haloplanctis A23 grown at 4 degrees C. In order to contribute to the understanding of the molecular basis of cold adaptations, crystallographic studies of this cold-adapted enzyme have been initiated because a three-dimensional structure of a mesophilic counterpart, pig pancreatic alpha-amylase, already exists. alpha-Amylase from A. haloplanctis, which shares 53% sequence identity with pig pancreatic alpha-amylase, has been crystallized and data to 1.85 A have been collected. The space group is found to be C222(1) with a = 71.40 A, b = 138.88 A, and c = 115.66 A. Until now, a three-dimensional structure of a psychrophilic enzyme is lacking.

摘要

从在4℃下培养的南极嗜冷菌嗜盐栖热袍菌A23的培养上清液中纯化出一种冷活性α-淀粉酶。为了有助于理解冷适应的分子基础，已经开始对这种冷适应酶进行晶体学研究，因为已经存在嗜温对应物猪胰α-淀粉酶的三维结构。与猪胰α-淀粉酶具有53%序列同一性的嗜盐栖热袍菌α-淀粉酶已经结晶，并收集到了分辨率为1.85 Å的数据。发现空间群为C222(1)，a = 71.40 Å，b = 138.88 Å，c = 115.66 Å。到目前为止，还缺乏嗜冷酶的三维结构。