萤火虫荧光素酶反应中荧光素腺苷酸合成的时间进程。

Time course of luciferyl adenylate synthesis in the firefly luciferase reaction.

作者信息

Dukhovich A, Sillero A, Sillero M A

机构信息

Departamento de Bioquímica, Instituto de Investigaciones Biomedicas del CSIC, Universidad Autónoma de Madrid, Spain.

出版信息

FEBS Lett. 1996 Oct 21;395(2-3):188-90. doi: 10.1016/0014-5793(96)01038-1.

DOI:10.1016/0014-5793(96)01038-1

PMID:8898092

Abstract

The time course of luciferyl adenylate formation in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been followed. The properties of luciferyl adenylate, enzymatically or chemically synthesized, as substrate of luciferase, have been compared. The potential use of luciferyl adenylate for luciferase detection is here proposed.

摘要

萤火虫荧光素酶（EC 1.13.12.7）催化的反应中，荧光素腺苷酸形成的时间进程已被跟踪。已比较了酶促合成或化学合成的荧光素腺苷酸作为荧光素酶底物的性质。本文提出了荧光素腺苷酸在荧光素酶检测中的潜在用途。