从菠菜叶绿体中分离出的CF1和ε-缺失CF1 ATP酶上，抗霉素A至少有两个结合位点。

Tentoxin has at least two binding sites on CF1 and epsilon-depleted CF1 ATPases isolated from spinach chloroplast.

作者信息

Pinet E, Gomis J M, Girault G, Cavelier F, Verducci J, Noël J P, André F

机构信息

Département de Biologie Cellulaire et Moléculaire, CEA-Saclay, Gif-sur-Yvette, France.

出版信息

FEBS Lett. 1996 Oct 21;395(2-3):217-20. doi: 10.1016/0014-5793(96)01043-5.

DOI:10.1016/0014-5793(96)01043-5

PMID:8898099

Abstract

A new procedure for synthesis of 14C-labeled tentoxin [14C-MePhe[(Z)delta]3-tentoxin], with a high specific activity, is described. Binding experiments with CF1 or CF1-epsilon isolated from spinach chloroplast have been carried out using equilibrium dialysis technique. The results show the presence of two classes of binding sites. The association constants of the two major binding sites were derived from non-linear fitting of the binding curves. At 4 degrees C, the first binding site has a value of Ka1 = 8.2 x 10(5) M(-1) in CF1 and 8.7 x 10(5) M(-1) in CF1-epsilon, while the second binding site has lower affinity with Ka2 = 1.5 x 10(4) M(-1) in CF1 and 2.3 x 10(3) M(-1) in CF1-epsilon.

摘要

本文描述了一种合成具有高比活度的14C标记抗霉素[14C-甲基苯丙氨酸[(Z)δ]3-抗霉素]的新方法。使用平衡透析技术对从菠菜叶绿体中分离出的CF1或CF1-ε进行了结合实验。结果显示存在两类结合位点。通过对结合曲线进行非线性拟合得出了两个主要结合位点的缔合常数。在4℃时，第一个结合位点在CF1中的Ka1值为8.2×10(5) M(-1)，在CF1-ε中的Ka1值为8.7×10(5) M(-1)，而第二个结合位点的亲和力较低，在CF1中的Ka2值为1.5×10(4) M(-1)，在CF1-ε中的Ka2值为2.3×10(3) M(-1)。