肿瘤坏死因子介导的人前列腺癌细胞系中的细胞毒性与耐药性
TNF-mediated cytotoxicity and resistance in human prostate cancer cell lines.
作者信息
Nakajima Y, DelliPizzi A M, Mallouh C, Ferreri N R
机构信息
Department of Urology, New York Medical College, Valhalla 10595, USA.
出版信息
Prostate. 1996 Nov;29(5):296-302. doi: 10.1002/(SICI)1097-0045(199611)29:5<296::AID-PROS4>3.0.CO;2-8.
BACKGROUND
The contribution of TNF receptor (TNF-R) expression was investigated with respect to TNF sensitivity or insensitivity for androgen-dependent and androgen-independent human prostate cancer (PCA) cell lines, respectively.
METHODS
Flow cytometric analyses using monoclonal antibodies against the 55-kDa receptor (TNF-R1) and the 75-kDa receptor (TNF-R2) indicated that both receptors were expressed on all three cell lines.
RESULTS
Moreover, expression of TNF-R1 was greater than expression of TNF-R2 in these PCA cells. All three PCA cell lines produced IL-6. However, IL-6 production was enhanced when TNF-insensitive JCA-1 and PC-3 cells, but not TNF-sensitive LNCaP cells, were treated with rTNF (10(-9) M).
CONCLUSIONS
These data suggest that the lack of an antiproliferative effect of rTNF on the androgen-independent PCA cell lines PC-3 and JCA-1 is not due to the failure of these cells to express TNF-R, but may be related to the differences in TNF-mediated IL-6 expression by these PCA cell lines.
背景
分别针对雄激素依赖性和雄激素非依赖性人前列腺癌细胞系（PCA）对肿瘤坏死因子（TNF）的敏感性或不敏感性，研究了TNF受体（TNF-R）表达的作用。
方法
使用针对55 kDa受体（TNF-R1）和75 kDa受体（TNF-R2）的单克隆抗体进行流式细胞术分析，结果表明这两种受体在所有三种细胞系上均有表达。
结果
此外，在这些PCA细胞中，TNF-R1的表达高于TNF-R2的表达。所有三种PCA细胞系均产生白细胞介素-6（IL-6）。然而，当用重组TNF（10⁻⁹ M）处理TNF不敏感的JCA-1和PC-3细胞而非TNF敏感的LNCaP细胞时，IL-6的产生增加。
结论
这些数据表明，重组TNF对雄激素非依赖性PCA细胞系PC-3和JCA-1缺乏抗增殖作用并非由于这些细胞未能表达TNF-R，而是可能与这些PCA细胞系中TNF介导的IL-6表达差异有关。
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