Kruse H, Heimann H, Schüler A, Lee W R, Hoffmann F
Department of Ophthalmology, Steglitz Medical Center, Free University of Berlin, Germany.
Cornea. 1996 Nov;15(6):622-32.
Keratokyphosis, a "nonfreeze keratomileusis" procedure, was applied in rabbits. The refractive changes were -6.5 +/- 0.7 (SD), -9.5 +/- 2.6, and -13.0 +/- 3.2 D for convex applanation surfaces with a vertex of +100 microns, +125 microns, and +150 microns in the microkeratome and +1.5 +/- 2.0 and +2.5 +/- 2.6 D for concave applanation surfaces with a vertex of -100 microns and -125 microns. The pachymetric corneal changes were +43 +/- 24 microns, +39 +/- 32 microns, and +71 +/- 24 microns for the convex applanation surfaces and -21 +/- 40 microns and -17 +/- 24 microns for the concave. Wound healing was uncomplicated. The scar at the interface was 0-5 microns wide. The mean central density of nerve endings was 1,255 +/- 532/mm2. The endothelial cell size was 25.1% (p < 0.01) greater as measured by specular microscopy than by vital preparation technique; this difference was greater with higher (33.4%) than with lower (18.02%) corneal refraction (p = 0.02). In addition, there was a method-independent correlation (correlation coefficient, -0.63) between endothelial cell count and corneal thickness. This may indicate an increased demand on corneal endothelium in thickened corneas.
在兔身上应用了一种“非冷冻角膜磨镶术”——角膜驼背术。对于角膜板层刀中顶点为 +100微米、+125微米和 +150微米的凸面压平表面，屈光变化分别为 -6.5±0.7（标准差）、-9.5±2.6和 -13.0±3.2D；对于顶点为 -100微米和 -125微米的凹面压平表面，屈光变化分别为 +1.5±2.0和 +2.5±2.6D。对于凸面压平表面，角膜厚度变化为 +43±24微米、+39±32微米和 +71±24微米；对于凹面压平表面，角膜厚度变化为 -21±40微米和 -17±24微米。伤口愈合过程顺利。界面处的瘢痕宽度为0 - 5微米。神经末梢的平均中央密度为1255±532/mm²。通过镜面显微镜测量的内皮细胞大小比活体制片技术测量的大25.1%（p < 0.01）；这种差异在角膜屈光较高（33.4%）时比屈光较低（18.02%）时更大（p = 0.02）。此外，内皮细胞计数与角膜厚度之间存在与方法无关的相关性（相关系数为 -0.63）。这可能表明增厚的角膜对角膜内皮的需求增加。
