Miul'berg A A, Tishchenko L I, Domkina L K
Biokhimiia. 1977 May;42(5):850-9.
Soluble fragments of chromatin obtained by Ca, Mg-dependent endonuclease digest of rat liver nuclei, have been separated by gel chromatography on Sepharose 4B into three zones, containing oligomers, tetramers--dimers and monomers, respectively. The content of nonhistone proteins and particularly lysine-rich histones is decreased with a transition from theoligomers to monomers. The average protein/DNA ratio of the monomers is equal to 1.36 and that of histone/DNA ratio--to 0.82. The dependence of the degree of chromatin digest by endonuclease on its protein content and conditions of isolation and incubation of nuclei is discussed. The chromatin monomer formed appears to be made up of a nucleosome and short portions of spacer DNA bound to some part of histone HI and nonhistone proteins.
通过用钙、镁依赖性核酸内切酶消化大鼠肝细胞核获得的可溶性染色质片段，已通过琼脂糖4B凝胶色谱法分离为三个区带，分别含有寡聚体、四聚体——二聚体和单体。随着从寡聚体到单体的转变，非组蛋白尤其是富含赖氨酸的组蛋白的含量降低。单体的平均蛋白质/DNA比值等于1.36，组蛋白/DNA比值为0.82。讨论了核酸内切酶对染色质的消化程度与其蛋白质含量以及细胞核分离和孵育条件的关系。所形成的染色质单体似乎由一个核小体和与组蛋白H1及非组蛋白的某些部分结合的间隔DNA短片段组成。
