Shimizu K, Satuito C G, Saikawa W, Fusetani N
Fusetani Biofouling Project, ERATO, JRDC, Yokohama R&D Center of Niigata Engineering Co., Ltd., Japan.
J Exp Zool. 1996 Oct 1;276(2):87-94. doi: 10.1002/(SICI)1097-010X(19961001)276:2<87::AID-JEZ1>3.0.CO;2-9.
Biochemical and immunological characterization of cyprid major protein (CMP), the principal protein constituent of cypris larvae of the barnacle Balanus amphitrite (Crustacea: Cirripedia), revealed similarities to egg-yolk protein, vitellin, as follows: CMP and vitellin heavy chain both have a molecular weight of 170 kDa by polyacrylamide gel electrophoresis containing sodium dodecylsulfate; CMP was crossreactive with antiserum against vitellin heavy chain in immunoblot analysis. The sequence of 11 amino acids in the amino-terminus of CMP, however, is not perfectly homologous to that of vitellin heavy chain. Thus, it was deduced that CMP was an isoform of vitellin. Concentration of CMP abruptly increased during the latter naupliar stages, reaching a peak just after metamorphosis to the non-feeding cypris stage, and decreased thereafter with aging of cyprids or during the early juvenile period. Specifically, the concentration of CMP in newly metamorphosed juveniles within one day decreased to 20% that of cyprids. CMP, therefore, appears to function as a storage protein during settlement of cyprids as well as metamorphosis to juveniles. Immunohistochemical analysis using antiserum against vitellin heavy chain on sectioned cyprids suggested that CMP is accumulated in the haemocoel.
藤壶白脊藤壶（甲壳纲：蔓足亚纲）金星幼虫主要蛋白质成分——金星幼虫主要蛋白（CMP）的生化和免疫特性研究表明，其与卵黄蛋白——卵黄磷蛋白存在相似之处，具体如下：在含有十二烷基硫酸钠的聚丙烯酰胺凝胶电泳中，CMP和卵黄磷蛋白重链的分子量均为170 kDa；在免疫印迹分析中，CMP与抗卵黄磷蛋白重链抗血清发生交叉反应。然而，CMP氨基末端的11个氨基酸序列与卵黄磷蛋白重链的序列并非完全同源。因此，推断CMP是卵黄磷蛋白的一种同工型。CMP的浓度在无节幼虫后期急剧增加，在变态为不摄食的金星幼虫阶段后立即达到峰值，此后随着金星幼虫的老化或在幼体早期阶段而降低。具体而言，新变态后一天内的幼体中CMP的浓度降至金星幼虫的20%。因此，CMP似乎在金星幼虫的附着以及变态为幼体的过程中起到储存蛋白的作用。使用抗卵黄磷蛋白重链抗血清对金星幼虫切片进行免疫组织化学分析表明，CMP在血腔中积累。
