Simultaneous recognition of ionocytes and mucous cells in the gill epithelium of turbot and in the rat stomach.

A simple and rapid method is described which allows the recognition of different types of epithelial cells in one paraffin section. In the fish gill, the "chloride cells" (also called ionocytes or mitochondria-rich cells) appeared black and well contrasted after the zinc iodideosmium (ZIO) fixation, a contrast which is preserved after staining with alcian blue. All mucous cells appeared pale beige and can be clearly distinguished from the other cells but the known specificity of alcian blue for acid and sulfate mucins was lost. A complex tissue like the gill showed an interesting metachromasia. The combined ZIO fixation and alcian blue staining has also been applied on another epithelium, the rat stomach.