Franzén B, Auer G, Alaiya A A, Eriksson E, Uryu K, Hirano T, Okuzawa K, Kato H, Linder S
Unit of Cell and Molecular Analysis, Department of Oncology and Pathology, Karolinska Institute and Hospital, Stockholm, Sweden.
Int J Cancer. 1996 Oct 21;69(5):408-14. doi: 10.1002/(SICI)1097-0215(19961021)69:5<408::AID-IJC10>3.0.CO;2-Z.
We describe the results from a protein-based approach to the study of heterogeneity in gene expression between human tumors. Cell preparations from 5 benign breast lesions, 5 potentially weakly malignant and 4 potentially highly malignant invasive ductal breast carcinomas were examined by 2-dimensional gel electrophoresis (2-DE) gels. Qualitative and quantitative differences were recorded by computerized analysis. Analysis of samples from different areas of the same tumor showed a high degree of similarity in the pattern of polypeptide expression. Analysis of 2 tumors and their metastases revealed similar 2-DE profiles. In contrast, variations between different lesions with comparable histological characteristics were considerable. Greater differences in polypeptide expression were observed between potentially highly malignant carcinomas compared with comparisons of less malignant lesions. Our results show that malignant human breast carcinomas may be highly heterogeneous in their patterns of gene expression.
我们描述了一种基于蛋白质的方法用于研究人类肿瘤之间基因表达异质性的结果。通过二维凝胶电泳（2-DE）凝胶对来自5个良性乳腺病变、5个潜在低度恶性和4个潜在高度恶性浸润性导管乳腺癌的细胞制剂进行了检测。通过计算机分析记录了定性和定量差异。对同一肿瘤不同区域的样本分析显示，多肽表达模式具有高度相似性。对2个肿瘤及其转移灶的分析揭示了相似的二维电泳图谱。相比之下，具有可比组织学特征的不同病变之间的差异相当大。与低度恶性病变相比，在潜在高度恶性癌之间观察到更大的多肽表达差异。我们的结果表明，恶性人类乳腺癌在基因表达模式上可能高度异质。
