Molnár J, Mándi Y, Földes J, Földeák S, Molnár M, Motohashi N
Faculty of Medicine, Albert Szent-Györgyi Medical University, Szeged, Hungary.
In Vivo. 1995 Sep-Oct;9(5):463-8.
The endotoxin neutralizing effects of several phenothiazines, benzophenothiazines and Pentaglobin (control) were investigated by spectrophotometry, tumor necrosis factor (TNF) induction and the conventional Limulus test. In animal experiments, some beneficial effects of complex forming compounds were found, however, the compounds could not completely inactivate the biological effect of endotoxin in the Limulus test. The complex formation between endotoxin and the compounds were revealed in spectrophotometry. The TNF inducing effect of compound-endotoxin complexes was markedly reduced by some phenothiazines and benzo[a]phenothiazines in leukocytes. Benz[c]acridines and Pentaglobin could not neutralize completely the TNF induction of E. coli endotoxin. The recent findings indicate that multifocal effects of phenothiazines and benzophenothiazines can be responsible for anti-endotoxin action in vivo. Hypotensive action in experimental animals was reduced by some phenothiazines in some preliminary experiments.
通过分光光度法、肿瘤坏死因子（TNF）诱导实验和传统鲎试剂检测法，研究了几种吩噻嗪类、苯并吩噻嗪类化合物以及Pentaglobin（对照）的内毒素中和作用。在动物实验中，发现形成复合物的化合物具有一些有益作用，然而，在鲎试剂检测中这些化合物不能完全灭活内毒素的生物学效应。分光光度法显示了内毒素与这些化合物之间形成了复合物。一些吩噻嗪类和苯并[a]吩噻嗪类化合物可显著降低化合物-内毒素复合物在白细胞中诱导TNF的作用。苯并[c]吖啶类化合物和Pentaglobin不能完全中和大肠杆菌内毒素诱导的TNF。最近的研究结果表明，吩噻嗪类和苯并吩噻嗪类化合物的多靶点作用可能是其在体内发挥抗内毒素作用的原因。在一些初步实验中，某些吩噻嗪类化合物可减轻实验动物的低血压作用。
