Brinck U, Stachura J, Rudzki Z, Kellner S, Hoefer K, Schauer A
Department of Pathology, Georg August University Göttingen, Germany.
In Vivo. 1995 Sep-Oct;9(5):475-8.
57 cases of malignant fibrous histiocytomas (MFH) were examined, immunohistochemically for the presence of p-53-tumour suppressor gene with monoclonal antibody DO-7 (Dako) and for the presence of Ki-67 antigen using monoclonal antibody MIB-1 (Dianova). The statistical relationship between the overexpression of p-53 and grading parameters (mitoses, cellularity, necrosis) and expression of Ki-67 antigen as well as survival time, was determined. The presence of p-53 antigen was found in 29.8% of grade II and grade III tumours, but not in MFH of grade I. The p-53 positive cases demonstrated a significantly higher mitotic index (p < 0.05), higher Ki-67 index (p < 0.05) and shorter survival time (p < 0.05) in comparison to p-53 negative cases. Differences concerning cellularity and extent of necrosis, between p-53 positive and p-53 negative tumours were not found (p > 0.05). The prognostic role of p-53 overexpression in MFH is discussed.
对57例恶性纤维组织细胞瘤（MFH）进行了检查，采用单克隆抗体DO-7（达科公司）免疫组化检测p-53肿瘤抑制基因的表达情况，采用单克隆抗体MIB-1（戴安诺娃公司）检测Ki-67抗原的表达情况。确定了p-53过表达与分级参数（有丝分裂、细胞密度、坏死）、Ki-67抗原表达以及生存时间之间的统计学关系。在Ⅱ级和Ⅲ级肿瘤中，29.8%检测到p-53抗原的存在，而Ⅰ级MFH中未检测到。与p-53阴性病例相比，p-53阳性病例的有丝分裂指数显著更高（p<0.05）、Ki-67指数更高（p<0.05）且生存时间更短（p<0.05）。未发现p-53阳性和p-53阴性肿瘤在细胞密度和坏死程度方面存在差异（p>0.05）。讨论了p-53过表达在MFH中的预后作用。
