Flurer C L
Forensic Chemistry Center, US Food and Drug Administration, Cincinnati, OH, 45202, USA.
Electrophoresis. 1996 Feb;17(2):359-66. doi: 10.1002/elps.1150170212.
Capillary electrophoresis was utilized in the study of the macrolide antibiotics (i.e. pharmaceutical glycoconjugates) clarithromycin, erythromycin, oleandomycin, troleandomycin, and spiramycin. In order to assist in analyte solubilization, two buffer systems using acetonitrile were developed. The first system involved 30 mM sodium cholate and 20% acetonitrile in 80 mM sodium phosphate, pH 6. This buffer permitted the baseline resolution of all five glycoconjugated antibiotics. In addition, erythromycin was separated from its derivatives estolate and ethylsuccinate. In the absence of surfactants, a higher acetonitrile quantity, 65%, was used in the second buffer system, with 35 mM sodium phosphate, pH 6. Selectivity between oleandomycin and clarithromycin was reversed in this system compared to the cholate buffer, indicating solute interaction with the cholate micelles in the previous system. Calibration linearity and detection sensitivity were improved in the high acetonitrile buffer, due to decreased background absorbance. It was demonstrated that both buffer systems can be utilized for the visualization of minor components that may be present in bulk pharmaceuticals.
毛细管电泳用于研究大环内酯类抗生素（即药物糖缀合物）克拉霉素、红霉素、竹桃霉素、醋竹桃霉素和螺旋霉素。为了帮助分析物溶解，开发了两种使用乙腈的缓冲系统。第一种系统在80 mM磷酸钠（pH 6）中含有30 mM胆酸钠和20%乙腈。这种缓冲液使所有五种糖缀合抗生素实现了基线分离。此外，红霉素与其衍生物依托红霉素和琥乙红霉素得以分离。在没有表面活性剂的情况下，第二种缓冲系统使用了更高含量的乙腈（65%），以及35 mM磷酸钠（pH 6）。与胆酸盐缓冲液相比，该系统中竹桃霉素和克拉霉素之间的选择性发生了逆转，表明在前一个系统中溶质与胆酸盐胶束发生了相互作用。由于背景吸光度降低，高乙腈缓冲液中的校准线性和检测灵敏度得到了提高。结果表明，两种缓冲系统均可用于可视化散装药物中可能存在的微量成分。
