Suzuki T, Sugimoto E, Tahara Y, Yamada Y
United Graduate School of Agricultural Science, Gifu University (Shizuoka University), Japan.
Biosci Biotechnol Biochem. 1996 Mar;60(3):444-7. doi: 10.1271/bbb.60.444.
The AgeI restriction-modification system from a marine bacterium, Agrobacterium gelatinovorum IAM 12617, recognizes the nucleotide sequence ACCGGT. The gene coding for the AgeI methylase (M.AgeI) was cloned into Escherichia coli DH5 alpha MCR, and the nucleotides of the gene were sequenced. The M.AgeI gene coded for a protein of 429 amino acid residues (molecular mass, 47,358 daltons). The deduced amino acid sequence of M.AgeI was compared with those of other methylases and showed that there are high degrees of similarity in some cytosine-5 methylases.
来自海洋细菌明胶土壤杆菌IAM 12617的AgeI限制修饰系统识别核苷酸序列ACCGGT。编码AgeI甲基化酶（M.AgeI）的基因被克隆到大肠杆菌DH5α MCR中，并对该基因的核苷酸进行了测序。M.AgeI基因编码一个由429个氨基酸残基组成的蛋白质（分子量为47358道尔顿）。将推导的M.AgeI氨基酸序列与其他甲基化酶的序列进行比较，结果表明在一些胞嘧啶-5甲基化酶中存在高度相似性。
