Yamasaki Y, Konno H
Research Institute for Bioresources, Okayama University, Kurashiki, Japan.
Biosci Biotechnol Biochem. 1996 Mar;60(3):511-2. doi: 10.1271/bbb.60.511.
When Mucor javanicus was cultured with castanospermine, two alpha-glucosidases (CS-I, II) were produced. The two enzymes were purified by two CM-cellulofine column chromatographies, and compared with the alpha-glucosidase (C) from a culture without castanospermine. The molecular weights of the three enzymes were calculated to be 97,000 (CS-I, II) and 94,000 (C) from SDS-PAGE. CS-II was similar in hydrolysis of alpha-1,4-linkage to C, but hydrolyzed alpha-1,2-, alpha-1,3-, and alpha-1,6-linkages at a lower rate than C. The Michaelis constant of CS-I for malto-oligosaccharides was several times as high as the constants of CS-II and C, but CS-I hydrolyzed nigerose at a faster rate than maltose.
用粟精胺培养爪哇毛霉时，可产生两种α-葡萄糖苷酶（CS-I、II）。这两种酶通过两次CM-纤维素柱色谱法进行纯化，并与未添加粟精胺的培养物中的α-葡萄糖苷酶（C）进行比较。通过SDS-PAGE计算出这三种酶的分子量分别为97,000（CS-I、II）和94,000（C）。CS-II在α-1,4-键的水解方面与C相似，但在水解α-1,2-、α-1,3-和α-1,6-键时的速率低于C。CS-I对麦芽低聚糖的米氏常数是CS-II和C的常数的几倍，但CS-I水解黑曲霉糖的速率比水解麦芽糖的速率快。
