大鼠冠状病毒在源自F344大鼠的肠道细胞系RCN-9中的复制。

Replication of rat coronaviruses in intestinal cell line, RCN-9, derived from F344 rats.

作者信息

Ohsawa K, Watanabe Y, Takakura A, Itoh T, Sato H

机构信息

Laboratory Animal Center for Biomedical Research, Nagasaki University School of Medicine, Japan.

出版信息

Exp Anim. 1996 Oct;45(4):389-93. doi: 10.1538/expanim.45.389.

DOI:10.1538/expanim.45.389

PMID:8902504

Abstract

To examine the susceptibility of the epithelial cell line to rat coronavirus (RCV), we inoculated sialodacryoadenitis virus and Parker's RCV into five cell lines; JTC-19, rat L2, LLC, RCN-9 and LBC cells originating in the lungs, intestines and mammary tumors of rodents. Both RCVs were replicated in LBC and RCN-9 cells, but not in the others. The infectivity titers of both RCVs grown in RCN-9 cells were significantly higher than those in LBC cells in every passage (2.5-3.9 log rate). Both RCVs replicated in LBC cells showed higher tropism to RCN-9 cells than to LBC cells, suggesting that RCN-9 cells are more suitable for the replication of RCVs than LBC cells. The RCN-9 cell line would be useful for the investigation of RCV infection in rodents.

摘要

为检测上皮细胞系对大鼠冠状病毒（RCV）的易感性，我们将涎泪腺炎病毒和帕克氏RCV接种到五种细胞系中；分别是源自啮齿动物肺、肠和乳腺肿瘤的JTC - 19、大鼠L2、LLC、RCN - 9和LBC细胞。两种RCV均能在LBC和RCN - 9细胞中复制，但在其他细胞中不能。在RCN - 9细胞中培养的两种RCV的感染滴度在每一代中均显著高于LBC细胞中的感染滴度（2.5 - 3.9对数率）。在LBC细胞中复制的两种RCV对RCN - 9细胞的嗜性均高于对LBC细胞的嗜性，这表明RCN - 9细胞比LBC细胞更适合RCV的复制。RCN - 9细胞系将有助于研究啮齿动物中的RCV感染。