Gaertner D J, Smith A L, Paturzo F X, Jacoby R O
Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut.
Arch Virol. 1991;118(1-2):57-66. doi: 10.1007/BF01311303.
Cell lines of rodent origin were tested for susceptibility to infection with rat coronavirus (RCV), including sialodacryoadenitis virus (SDAV) and Parker's rat coronavirus (PRCV). LBC rat mammary adenocarcinoma cells were susceptible only if the cells were treated with diethylaminoethyl-dextran (DEAE-D). A recent report that RCVs grow well in L2 mouse fibroblast cells was confirmed and expanded. RCV infection of L2 cells was substantially enhanced by treatment of cells with trypsin but not by treatment with DEAE-D. Primary isolation of SDAV from experimentally infected rats was accomplished using trypsin-treated L2 cells. One of 13 additional cell lines tested (rat urinary bladder epithelium, RBL-02) supported growth of RCVs, and growth was slightly enhanced by DEAE-D, but not by trypsin. These refinements of in vitro growth conditions for RCVs should facilitate further studies of their basic biology and improve options for primary isolation.
对源自啮齿动物的细胞系进行了对大鼠冠状病毒(RCV)感染的易感性测试,包括涎泪腺炎病毒(SDAV)和帕克大鼠冠状病毒(PRCV)。LBC大鼠乳腺腺癌细胞只有在用二乙氨基乙基葡聚糖(DEAE-D)处理后才易感。最近一份关于RCV在L2小鼠成纤维细胞中生长良好的报告得到了证实并有所扩展。用胰蛋白酶处理细胞可显著增强L2细胞对RCV的感染,但用DEAE-D处理则无此效果。使用经胰蛋白酶处理的L2细胞从实验感染的大鼠中成功进行了SDAV的初次分离。在测试的另外13种细胞系中,有一种(大鼠膀胱上皮细胞,RBL-02)支持RCV的生长,DEAE-D可使其生长略有增强,但胰蛋白酶无此作用。这些对RCV体外生长条件的改进应有助于对其基础生物学的进一步研究,并改善初次分离的选择。