Gorbach V I, Krasikova I N, Lukyanov P A, Razmakhnina O Y, Solov'eva T F, Ovodov Y S
Eur J Biochem. 1979 Jul;98(1):83-6. doi: 10.1111/j.1432-1033.1979.tb13163.x.
Lipid A isolated from lipopolysaccharide of Yersinia pseudotuberculosis was used for immunization of rabbits to afford antisera to lipid A with titers of 1:640 in the passive hemolysis test. Exhaustion of immune serume with sheep erythrocytes decreased antibody titers up to 1:160. Authentic samples of 2-(DL-3-hydroxytetradecanoyl)amino-2-deoxy-D-glucose 6-phosphate, 2-tetradecanoylamino-2-deoxy-D-glucose 6-phosphate and 2-acetamido-2-deoxy-D-glucose 6-phosphate have been synthesized in order to carry out a comparative study of inhibitory activity of these compounds and lipid A using a system of lipid A and antiserum to lipid A. As a result, the immunodominant moiety of the lipid A of Y. pseudotuberculosis proved to contain a D-glucosamine residue acylated with 3-hydroxytetradecanoic acid at the amino group. The nature of the fatty acid acylating the amino group of glucosamine does not play an important role in the structure of immunodominant moiety of lipid A.
从假结核耶尔森氏菌脂多糖中分离出的类脂A用于免疫兔子，以获得在被动溶血试验中效价为1:640的抗类脂A抗血清。用绵羊红细胞耗尽免疫血清可使抗体效价降至1:160。为了使用类脂A和抗类脂A抗血清系统对这些化合物和类脂A的抑制活性进行比较研究，已合成了2-(DL-3-羟基十四烷酰基)氨基-2-脱氧-D-葡萄糖6-磷酸、2-十四烷酰氨基-2-脱氧-D-葡萄糖6-磷酸和2-乙酰氨基-2-脱氧-D-葡萄糖6-磷酸的真实样品。结果表明，假结核耶尔森氏菌类脂A的免疫显性部分在氨基处含有一个被3-羟基十四烷酸酰化的D-葡萄糖胺残基。酰化葡萄糖胺氨基的脂肪酸性质在类脂A免疫显性部分的结构中不起重要作用。
