Nouri A M, Hussain R F, Dos Santos A V, Oliver R T
Department of Medical Oncology, The Royal London Hospital, UK.
Urol Int. 1996;56(1):6-12. doi: 10.1159/000282799.
The pattern of expression of cell adhesion molecules, i.e., leucocyte function associated antigen 3 (LFA-3) and intercellular adhesion molecule 1 (ICAM-1) on human bladder tumour biopsy specimens was investigated. Attempts were also made to study the pattern of induction of these molecules by established human cell lines in response to cytokines. The results indicated that 15 of 25 tumour biopsy specimens were negative for ICAM-1, and amongst the remaining 10, only 1 showed strong positivity, whilst LFA-3 was expressed in 21 of 23 cases. Unlike LFA-3, the pattern of ICAM-1 expression on established tumour cell lines was different in that there were 7 of 21 cases showing positive staining. The parallel investigation of ICAM-1 and major histocompatibility complex class II antigen expression on bladder tumours showed that in 11 of 18 cases, there was a concomitant expression or complete absence of these molecules. In the remaining 7, there were 6 cases where only class II expression was observed. Exposure of cell lines to interferons alpha or gamma had no effects on LFA-3 expression. In contrast, interferon gamma induced ICAM-1 on all the eight lines with constitutive ICAM-1 expression, whereas interferon alpha upregulated only 2 of these 8 lines. The mean +/- SD values for ICAM-1 expression on the eight inducible lines were 617 +/- 406 cpm before and 943 +/- 471 cpm (p = 0.001) after interferon gamma stimulation. The pattern of ICAM-1 inducibility of a bladder cell line Fen to interferon remained unchanged following transfection of a beta2-microglobulin gene and correction of cell surface HLA class I antigens. These results indicate that there was a significant minority of bladder tumours and tumour cell lines with abnormal cell adhesion molecule expression. In some cases, the abnormality in cell lines could not be corrected by cytokine stimulation. It is possible that these abnormalities may play a critical role in the overall tumour strategy for escape from immunological detection.
研究了人膀胱肿瘤活检标本上细胞黏附分子，即白细胞功能相关抗原3（LFA - 3）和细胞间黏附分子1（ICAM - 1）的表达模式。还尝试研究已建立的人细胞系在细胞因子作用下这些分子的诱导模式。结果表明，25例肿瘤活检标本中有15例ICAM - 1呈阴性，其余10例中只有1例呈强阳性，而23例中有21例表达LFA - 3。与LFA - 3不同，已建立的肿瘤细胞系上ICAM - 1的表达模式有所不同，21例中有7例呈阳性染色。对膀胱肿瘤上ICAM - 1和主要组织相容性复合体II类抗原表达的平行研究表明，18例中有11例这些分子同时表达或完全缺失。其余7例中，有6例仅观察到II类表达。细胞系暴露于α或γ干扰素对LFA - 3表达无影响。相比之下，γ干扰素在所有8个组成型表达ICAM - 1的细胞系上诱导ICAM - 1表达，而α干扰素仅上调这8个细胞系中的2个。8个可诱导细胞系上ICAM - 1表达的平均±标准差在γ干扰素刺激前为617±406 cpm，刺激后为943±471 cpm（p = 0.001）。膀胱细胞系Fen对干扰素的ICAM - 1诱导模式在转染β2 - 微球蛋白基因并校正细胞表面HLA I类抗原后保持不变。这些结果表明，相当一部分膀胱肿瘤和肿瘤细胞系存在细胞黏附分子表达异常。在某些情况下，细胞系中的异常不能通过细胞因子刺激来纠正。这些异常可能在肿瘤逃避免疫检测的整体策略中起关键作用。
