Killinger S, Blume G L, Bohart L, Bested A, Dias L S, Cooper B, Allan R D, Balcar V J
Department of Anatomy and Histology, University of Sydney, Australia.
Neurosci Lett. 1996 Sep 27;216(2):101-4. doi: 10.1016/0304-3940(96)13015-9.
Quantitative autoradiography of [3H]L-aspartate binding in thaw-mounted sections of rat brain has shown that L-trans-pyrrolidine-2,4-dicarboxylate and D-threo-3-hydroxyaspartate but not DL-2 aminoadipate strongly interacted with the binding sites while dihydrokainate, kainate and beta-aminoadipate produced only weak effects. The potency of inhibitors did not vary from one region to another in the telencephalon (neocortex, hippocampus and neostriatum) but, D-threo-3-hydroxyaspartate, L-trans-pyrrolidine-2,4-dicarboxylate, kainate and dihydrokainate inhibited [3H]L-aspartate binding in the cerebellar cortex less potently than that in the forebrain. Characteristics of the known excitatory amino acid transporters can, in part, explain the present results but contributions from additional transporter molecules to the heterogeneity of [3H]L-aspartate binding sites cannot be ruled out.
对大鼠脑解冻切片中[3H]L-天冬氨酸结合进行的定量放射自显影显示，L-反式吡咯烷-2,4-二羧酸和D-苏式-3-羟基天冬氨酸能与结合位点强烈相互作用，而DL-2-氨基己二酸则不能，同时二氢海人藻酸、海人藻酸和β-氨基己二酸仅产生微弱影响。在端脑（新皮质、海马体和新纹状体）中，抑制剂的效力在不同区域间并无差异，但是，D-苏式-3-羟基天冬氨酸、L-反式吡咯烷-2,4-二羧酸、海人藻酸和二氢海人藻酸对小脑皮质中[3H]L-天冬氨酸结合的抑制效力低于对前脑的抑制效力。已知兴奋性氨基酸转运体的特性可以部分解释目前的结果，但不能排除其他转运体分子对[3H]L-天冬氨酸结合位点异质性的作用。
